Hybrid rice (Oryza sativa L.): identification and parentage determination by RAPD fingerprinting |
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Authors: | Gejiao Wang Stefano Castiglione Jian Zhang Rongzhao Fu Jiangsheng Ma Wenbin Li Yongru Sun Francesco Sala |
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Institution: | (1) Consorzio Nazionale Biologia Molecolare delle Piante, sez. Pavia c/o Department of Genetics and Microbiology, University of Pavia, Via Abbiategrasso 207, 27100 Pavia, Italy;(2) Institute of Genetics, Academia Sinica, 100101 Beijing, China |
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Abstract: | Summary DNA from three families of rice plants selected in Northern China (each comprising the male sterile, the restorer, the hybrid F1 and the maintainer lines) has been extracted and amplified by PCR with different random DNA primers (RAPD analysis). Then, DNA has been analysed by agarose gel electrophoresis and DNA bands scored as present or absent. The generated matrices are reproducible and amenable for identification of each single plant line. Thus, RAPD fingerprinting of the inbred parental lines and of the resulting hybrid is proposed as a convenient tool for the identification, protection and parentage determination of plant hybrids. Furthermore, by offering a molecular tool to verify the degree of dissimilarity between the parental lines, the RAPD analysis may also be used to search for new parental combinations. |
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Keywords: | Hybrid rice RAPD PCR Oryza sativa DNA fingerprinting Molecular markers |
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