Pyrimidine-specific chemical reactions useful for DNA sequencing. |
| |
Authors: | C M Rubin and C W Schmid |
| |
Abstract: | Potassium permanganate reacts selectively with thymidine residues in DNA (1) while hydroxylamine hydrochloride at pH 6 specifically attacks cytosine (2). We have adopted these reactions for use with the chemical sequencing method developed by Maxam and Gilbert (3). |
| |
Keywords: | |
|
|