Enucleation of CHO cells by means of cytochalasin B and centrifugation: the topography of enucleation.

The lanthanum colloidal technique has been used for studying in electron microscopy the modifications occurring during blast transformation in the composition and the amounts of the lanthanum-retaining surface components of human blood T and B lymphocytes. The transformation event has usually been followed by a slight decrease in the surface labeling of the T-line lymph cells. Conversely, in the B-derived lymphocyte population various important staining alterations have been observed.The biochemical meaning of the above-mentioned differential alteration occurring in the lanthanum-staining of the stimulated lymphocyte's surface as a function of their T or B origin is further discussed.