DNA amounts in the nuclei ofParamecium aurelia andTetrahymena pyriformis |
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Authors: | Dr. Ian Gibson Dr. Norman Martin |
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Affiliation: | (1) School of Biological Sciences, University of East Anglia, University Plain, NOR 88C Norwich, England |
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Abstract: | DNA amounts have been determined in the micronuclei and macronuclei of 8 strains ofParamecium aurelia and 6 strains ofTetrahymena pyriformis. In the case ofTetrahymena a distribution of values for the amount of DNA in the macronuclei of all the strains was observed but the lowest values were approximately the same, viz. 1.17×10−11 g. There are two groups of strains in relation to micronuclear DNA values ofTetrahymena, one giving an average of 0.36×10−12 g and the other 0.815×10−12 g. The ratio of MIC/MAC DNA varies in the two groups.Paramecium again has a range of macronuclear values within each stock—lowest value 2.51×10−10 g—and the micronuclear values are similar in all stocks—approximately 0.613×10−12 g. The ratio of MIC/MAC DNA is similar in each stock.—The haploid genome values calculated from these data show excellent agreement with the values obtained by DNA renaturation studies. Supported by a Research Grant B/SR/8276 from the Science Research Council. The Vickers densitometer was purchased with a grant from the Medical Research Council. |
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