Kinetics of ethanol production by immobilized cells of Zymomonas mobilis at varying d-glucose concentrations |
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Authors: | Pramod K. Bajpai Argyrios Margaritis |
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Affiliation: | Chemical and Biochemical Engineering, Faculty of Engineering Science, University of Western Ontario, London, Ontario, Canada N6A 5B9 |
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Abstract: | Ethanol fermentation by cells of Zymomonas mobilis immobilized in calcium alginate gel has been studied using 5 to 30 wt% initial d-glucose in the medium. Up to 27% d-glucose was completely fermented and the maximum ethanol concentration of 12.6% (w/v) was obtained using an immobilized cell concentration of 58 g dry wt l?1 of bead volume. The ethanol yield coefficient was almost unaffected by initial d-glucose concentration and its value was >95% of theoretical. The rates of ethanol production and d-glucose utilization first increased, with an increase in initial d-glucose concentration up to 13.6%, and then started to decrease upon a further increase in initial d-glucose concentration. Cell leakage from the calcium alginate beads was very low. |
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Keywords: | Ethanol fermentation whole cell immobilization calcium alginate high ethanol concentration |
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