A rapid whole-mount staining procedure for nuclei of mammalian embryos |
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| Authors: | V.G. Pursel R.J. Wall C.E. Rexroad R.E. Hammer R.L. Brinster |
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| Affiliation: | 1. Reproduction Laboratory, Agricultural Research Service, USDA Beltsville, MD 20705 USA;2. Laboratory of Reproductive Physiology School of Veterinary Medicine University of Pennsylvania Philadelphia, PA 19104 USA |
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| Abstract: | A rapid staining procedure for examining nuclei of mammalian embryos is described. Embryos are placed on a glass slide, counterstained with trypan blue, stained with Hoechst 33342, and embedded between the slide and coverslip in Permount. The nuclei of the embryos fluoresce brightly when examined by fluorescence microscopy immediately after staining or after extended storage. The technique has proved to be an effective tool for studying the development of cow, hamster, mouse, pig, rabbit, and sheep embryos. |
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| Keywords: | Hoechst 33342 nuclear stain embryos trypan blue fluorescence |
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