水蛭常见病原菌的分离与鉴定 |
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引用本文: | 磨美兰,韦平,周维官,廖品静,陶锦华. 水蛭常见病原菌的分离与鉴定[J]. 动物学杂志, 2003, 38(3): 2-7 |
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作者姓名: | 磨美兰 韦平 周维官 廖品静 陶锦华 |
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作者单位: | 1. 广西大学动物科学技术学院,南宁,530005 2. 广西科学院生物研究所,南宁,530003 |
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摘 要: | 从表现软体、水肿、出血和硬结等特征的患病宽体金线蛭(Whitmania pigra)中分离到13株细菌分离物。通过培养特征、形态学观察及生化试验,确定了所分离的细菌中有6株为大肠杆菌(占46.15%),4株为变形杆菌(占30.77%),3株为沙门氏菌(占23.08%)。动物回归试验表明,接种后10d内大肠杆菌、沙门氏菌和变形杆菌对水蛭的致死率分别为100%(4/4)、100%(4/4)和25%(1/4);药敏试验结果表明,这三种细菌普通对复合磺胺、氯霉素、丁胺卡那霉素、头孢哌酮、丙氟哌酸、氟哌酸敏感。研究结果表明,本病是由多个病因导致的一种临床综合症。因此,在进行药物治疗时,应选用多种药物联合以达到最佳的治疗效果。
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关 键 词: | 水蛭 药敏试验 生化试验 回归试验 大肠杆菌 变形杆菌 沙门氏菌 |
文章编号: | 0250-3263(2003)03-02-06 |
修稿时间: | 2002-10-10 |
Isolation and Characterization of Common Pathogens from Diseased Leeches |
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Abstract: | bacteria were isolated from the diseased leeches(Witmania pigra),characterized by a soft body,edema and bleeding or hard abscess on the skin.Morphological features and the results of a biochemical test with bacterial isolates revealed that 6,4 and 3 bacteria were classified as Escherichia coli(constitutes 46^15% of the total isolates),Proteus (30^77%) and Salmonella(23^08%) respectively.A pathogenicity test on leeches found that mortality was 100%(4/4),100%(4/4) and 25%(1/4) respectively within 10 days post-inoculation with isolates of Escherichia coli,Salmonella and Proteus respectively.The drug susceptibility test showed that 3 pathogens were all highly sensitive to co-trimoxazole,chloramphenical,amikacin,cefoperazone,ciprofloxacin and norfiaxacin.Since the poor condition of the leeches was due to a multi-factorial rather than a simple mono-factor disease,a combination drug treatment is probably neccesary to cure the disease. |
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Keywords: | Leech(Whitmania pigra) Drug susceptivity test Biochemistry test Pathogenicity test Escherichia coli Proteus Salmonella |
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