Preservation of polyclonal antibody production by hybridization |
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| Authors: | Richard A. Goldsby Barbar A. Osborne Dipa Suri Joann Williams Adrian D. Mandel |
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| Affiliation: | (1) Department of Chemistry, University of Maryland, 20742 College Park, Maryland, USA;(2) Department of Genetics, Stanford University, 94305 Stanford, California, USA;(3) The Ames Research Center, 94305 Moffett Field, California, USA |
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| Abstract: | The fusion of unimmunized (Balb/c × SJL)F1, mouse spleen cells in which a polyclonal response had been induced by bacterial lipopolysaccharide with a myeloma cell line resulted in hybrid cell populations. The hybrid populations obtained elaborated antibody activity to human hemoglobin A, Keyhole Limpet hemocyanin, the dinitrophenyl (DNP) hapten, and human erythrocytes, Thus, hybridization allowed preservation of the normally transitory polyclonal response induced in mouse B cells by lipopolysaccharide. Furthermore, monospecific production of anti-DNP antibody was successfully factored out of the polyspecific production of antibodies by cloning. The expansion and subsequent injection of one of these clones into a (Balb/c × SJL)F1 mouse resulted in the formation of an antibody-producing tumor that was successfully passed to other (Balb/c × SJL)F1 recipients. Collection of the serum from tumor-bearing mice provided useful quantities of an anti-DNP antiserum without resort to any program of immunization whatsoever. |
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