免疫共沉淀结合微流控芯片技术筛选GBLP相互作用蛋白

为了进一步阐明G蛋白β亚基2样1蛋白（guanine nucleotide-binding protein subunit beta 2-like 1，GBLP）在普萘洛尔（propranolol，PRO）生物学效应发生机制中的作用，采用免疫共沉淀法结合液相色谱-芯片-离子阱串联质谱（HPLC-CHIP-IT-MS/MS）系统筛选并鉴定人脐静脉内皮细胞（human umbilical vein endothelial cell，HUVEC）中的GBLP相互作用蛋白.结果表明，有7种蛋白与GBLP存在相互作用，分别为酪蛋白α-S1、酪蛋白α-S2、β酪蛋白、桥粒芯蛋白1前体、α-烯醇化酶、果糖二磷酸醛缩酶C、硫氧还原蛋白过氧化物酶2.这些蛋白的生物信息学检索结果提示，GBLP可能参与了细胞的能量代谢调节和抗氧化机制，与普萘洛尔的生物学效应发生机制密切相关.

Screening of GBLP interacting Proteins with Co immunoprecipitation and an Integrated Microfluidic System

To further elucidate the biological characteristics and the roles of guanine nucleotide binding protein subunit beta 2-like 1 (GBLP）in response to propranolol (PRO)，co-immunoprecipitation and HPLC-CHIP-IT-MS/MS were used to screen and identity the GBLP interacting proteins in human umbilical vein endothelial cells.Seven proteins were shown to interact with GBLP，including casein α-S1，casein α-S2，β casein，desmoglein 1 precursor，α-enolase，fructose-bisphosphate aldolase C and peroxiredoxin 2.These proteins actively involved in energy metabolism of the cell，suggesting that GBLP might participated in the regulation of antioxidation processes，which correlated with the known biological functions of PRO.