Methyl Oleate Modulates <Emphasis Type="Italic">LIP2</Emphasis> Expression in the Lipolytic Yeast <Emphasis Type="Italic">Yarrowia lipolytica</Emphasis>期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

Methyl Oleate Modulates LIP2 Expression in the Lipolytic Yeast Yarrowia lipolytica

Authors:	Patrick?Fickers author-information" > author-information__contact u-icon-before" > mailto:p.thonart@ulg.ac.be" title=" p.thonart@ulg.ac.be" itemprop=" email" data-track=" click" data-track-action=" Email author" data-track-label=" " >Email author,Jacqueline?Destain,Philippe?Thonart

Affiliation:	(1) Centre Wallon de Biologie Industrielle, Service de Technologie Microbienne, Université de Liège, Bd du Rectorat Bat. 40, B-4000 Liège, Belgium;(2) Centre Wallon de Biologie Industrielle, Unité de Bio-industries, Faculté Universitaire des Sciences Agronomiques de Gembloux, Passage des Déportés 2, 5030 Gembloux, Belgium

Abstract:	Methyl oleate was used as a primary carbon source and as an alternative inducer for the production of an extracellular lipase, Lip2, in Y. lipolytica strain LgX64.81 grown in a 20-l bioreactor. The lipase-encoding gene, LIP2, was investigated during culture on methyl oleate using a pLIP2–LacZ reporter fusion and we provide evidence for the involvement of methyl oleate in its regulation. Revisions requested 7 July 2005; Revisions received 30 August 2005

Keywords:	bioreactor β -galactosidase LIP2 regulation lipase methyl oleate
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