Analysis of leaf proteins in late flowering mutants of Arabidopsis thaliana |
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Authors: | P. Tacchini A. Fink H. Thiellement H. Greppin |
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Affiliation: | Univ. of Geneva, Pavilion des Isotopes, 20, bvd. ďYvoy, CH-1211 Geneva 4, Switzerland. |
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Abstract: | Late flowering monogenic mutants of Arabidopsis thaliana (L.) Heynh. at the loci co, gi, fca, fve, fwa, fha, fpa, fy and their corresponding wild type, Landsberg erecta , were analysed by two-dimensional gel electrophoresis. All plants were grown under continuous light and proteins were extracted from leaves of the same age (20-day-old). The polypeptide patterns of the mutants at the loci co, gi, fca, fve, fwa, fha, fpa , and Landsberg erecta were identical. The mutant at the fy locus showed a qualitative difference with Landsberg erecta . Crosses were made between this line and the wild type Landsberg erecta . F2 plants, resulting from autopollination of the hybrid, were analysed and showed no cosegregation between the observed protein and the flowering phenotype, indicating that these two lines differ by more than a single mutation. |
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Keywords: | Arabidopsis thaliana late flowering mutants two-dimensional gel electrophoresis |
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