Modo-UG, a marsupial nonclassical MHC class I locus |
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Authors: | Nicolas Gouin April M. Wright Katarzyna B. Miska Zuly E. Parra Paul B. Samollow Michelle L. Baker Robert D. Miller |
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Affiliation: | (1) Department of Biology, The University of New Mexico, Albuquerque, NM 87131, USA;(2) Department of Genetics, Southwest Foundation for Biomedical Research, San Antonio, TX 78245, USA;(3) USDA-ARS, Animal Parasitic Diseases Laboratory, 10300 Baltimore Avenue, Building 1042 BARC-East, Beltsville, MD 20705, USA;(4) Department of Veterinary Integrative Biosciences, Texas A&M University, College Station, TX 77843, USA |
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Abstract: | Modo-UG is a class I gene located in the MHC of the marsupial Monodelphis domestica, the gray, short-tailed opossum. Modo-UG is expressed as three alternatively spliced mRNA forms, all of which encode a transmembrane form with a short cytoplasmic tail that lacks phosphorylation sites typically found in classical class I molecules. The three alternative mRNAs would encode a full-length form, an isoform lacking the α2 domain, and one lacking both α2 and α3 domains. Genotyping both captive-bred and wild M. domestica from different geographic regions revealed no variation in the residues that make up Modo-UG’s peptide-binding groove. Modo-UG’s low polymorphism is contrasting to that of a nearby class I locus, Modo-UA1, which has a highly polymorphic peptide-binding region. Absence of functional polymorphism in Modo-UG is therefore not a general feature of opossum class I genes but the result of negative selection. Modo-UG is the first MHC linked marsupial class I to be described that appears to clearly have nonclassical features.Electronic Supplementary Material Supplementary material is available for this article at |
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