A protocol for the efficient screening of putatively transformed plants forbar,the selectable marker gene,using the polymerase chain reaction |
| |
| Authors: | Joan E. Vickers Glenn C. Graham Robert J. Henry |
| |
| Affiliation: | (1) Department of Biochemistry, University of Queensland, 4072 St. Lucia, QLD;(2) CRC for Tropical Pest Management, Gehrmann Laboratories, University of Queensland, 4072 St. Lucia, QLD;(3) Centre for Plant Conservation Genetics, Southern Cross University, 2480 Lismore, NSW, Australia;(4) Present address: CSIRO Division of Tropical Agriculture, 4072 St. Lucia, Queensland, Australia |
| |
| Abstract: | Amplification of thebar gene usingTaq DNA polymerase in PCR is often not successful, possibly due tobar's high GC content. We describe a PCR protocol in which reliable amplification at a sensitivity of one gene copy per genome (in this study, barley) present in the reaction was achieved using a novel pair of primers and Expandtm High Fidelity DNA polymerase mix (Boehringer Mannheim). This method should allow for rapid screening of plants putatively transformed withbar. |
| |
| Keywords: | bar gene barley PCR screening transformation |
| 本文献已被 SpringerLink 等数据库收录！ |
|
