| 利用栽培稻C0t-1 DNA和基因组DNA比较分析宽叶野生稻基因组 |
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| 引用本文： | 吴绮,覃瑞,李刚,刘虹.利用栽培稻C0t-1 DNA和基因组DNA比较分析宽叶野生稻基因组[J].武汉植物学研究,2010,28(6):654-659. |
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| 作者姓名： | 吴绮 覃瑞 李刚 刘虹 |
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| 作者单位： | 中南民族大学生命科学学院南方少数民族地区生物资源保护与综合利用联合工程中心; |
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| 基金项目： | 中南民族大学自然科学基金 |
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| 摘 要： | 利用AA染色体组栽培稻的中高度重复序列C0t-1 DNA和基因组DNA作为探针,通过荧光原位杂交技术对宽叶野生稻（Oryza latifolia）（CCDD染色体组）进行了比较基因组分析。结果显示,在宽叶野生稻染色体上,C0t-1 DNA的杂交信号没有基因组DNA的杂交信号明显;杂交信号主要分布在着丝粒、近着丝粒及端粒区域;随着洗脱严谨度的不同,杂交信号呈现出较高的种特异性。本研究以不同洗脱严谨度下的荧光原位杂交结果为依据,对宽叶野生稻进行的核型分析,可进一步提高稻属染色体识别的准确性。
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| 关 键 词： | C0t-1DNA 基因组DNA 荧光原位杂交 洗脱严谨度 宽叶野生稻 |
Comparative Analysis of Oryza latifolia Genome with C0t-1 DNA and Genomic DNA of Oryza sativa |
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| WU Qi,QIN Rui,LI Gang,LIU Hong.Comparative Analysis of Oryza latifolia Genome with C0t-1 DNA and Genomic DNA of Oryza sativa[J].Journal of Wuhan Botanical Research,2010,28(6):654-659. |
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| Authors: | WU Qi QIN Rui LI Gang LIU Hong |
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| Institution: | WU Qi,QIN Rui,LI Gang,LIU Hong(Engineering Research Centre for the Protection and Utilization of Bioresource in Ethnic Area of Southern China,College of Life Sciences,South-Central University for Nationalities,Wuhan 430074,China) |
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| Abstract: | C0t-1 DNA and genomic DNA(gDNA) of cultivated rice Oryza sativa were used as probes to analyze comparatively the Oryza latifolia genome by fluorescence in situ hybridization(FISH).Results showed that the hybridization signals of gDNA were more distinct on the chromosomes of O.latifolia than that of C0t-1 DNA.These hybridization signals were mostly distributed on centromeres and telomeres regions as well as those near centromeres under different stringencies.With increasing washing stringency,hybridization s... |
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| Keywords: | C0t-1 DNA Genomic DNA FISH Stringency Oryza latifolia |
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