Transplantation of poly(<Emphasis Type="Italic">N</Emphasis>-isopropylacrylamide-co-vinylimidazole) hydrogel constructs composed of rabbit chondrocytes and growth factor-loaded nanoparticles for neocartilage formation |
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Authors: | Keun-Hong Park Don Haeng Lee Kun Na |
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Institution: | (1) Cell and Gene Therapy Research Institute, College of Medicine, Pochon CHA University, 606-16, Yeoksam 1-dong, Kangnam-gu, Seoul, 135-081, South Korea;(2) Center for Advanced Medical Education, Inha University College of Medicine by BK-21 Project, Incheon, South Korea;(3) Division of Biotechnology, The Catholic University of Korea, 43-1 Yokkok2-dong, Wonmi-gu, Bucheon, 420-743, South Korea |
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Abstract: | To evaluate their protein activity, heparinized nanoparticles (NPs) in which growth factors were loaded into a thermoreversible
hydrogel poly(N-isopropylacrylamide-co-vinylimidazole)]; p(NiPAAm-co-VI) have been investigated with regard to their activity in cell differentiation.
Specifically, rabbit chondrocytes were embedded in composite hydrogels co-encapsulating NPs loaded with transforming growth
factor β-1 (TGF β-1). The specific ECMs associated cartilage tissue component was determined via immunohistochemistry (IHC)
and Alcian blue (GAG) staining. In the same period of transplantation, the DNA content was different for all formulations,
thereby indicating that the dramatic increase in cell number for the TGF β-1 loaded NP samples was accompanied by the maintenance
of the cell phenotypes. These results suggested the growth factor-loaded heparinized NPs in a chondrocyte-embedded hydrogel
as suitable model for the cartilage tissue regeneration. |
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Keywords: | Poly(N-isopropylacrylamide-co-vinylimidazole) p(NiPAAm-co-VI) Transforming growth factor β -1 Chondrocytes Cartilage tissue engineering |
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