Gangliosides are Transported from the Plasma Membrane to Intralysosomal Membranes as Revealed by Immuno-Electron Microscopy |
| |
Authors: | W Möbius V Herzog K Sandhoff G Schwarzmann |
| |
Institution: | (1) Kekulé-Institute für Organische Chemie und Biochemie, Gerhard-Domagk-Strasse 1, 53121 Bonn, Germany. schwarzmann@uni-bonn.de;(2) Institut für Zellbiologie der Universität Bonn, 53121 Bonn, Germany |
| |
Abstract: | A biotin-labeled derivative of the ganglioside GM1 (biotin-GM1) was used to study its transport along the endocytic pathway of cultured fibroblasts by immuno-electron microscopy. Using electron dense endocytic tracers we could demonstrate that late endosomes and lysosomes of these cells are long living organelles with a high content of internal membranes. Our studies show that during endocytosis the biotin-GM1 was transported to these intraendosomal and intralysosomal membranes. These observations support the hypothesis that glycosphingolipids (GSL) are preferentially degraded in intralysosomal vesicles. |
| |
Keywords: | Biotin-GM1 immuno-electron microscopy ganglioside transport endocytosis intralysosomal membranes |
本文献已被 PubMed SpringerLink 等数据库收录! |