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Comparative mineral and hormonal analyses of wild type and TLS somaclonal variant derived from oil palm (Elaeis guineensis Jacq. var. tenera) tissue culture |
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| Authors: | S H Habib S-E Ooi Ond?ej Novák Danu?e Tarkowská Jakub Rol?ík Karel Dole?al S S R Syed-Alwee C-L Ho P Namasivayam |
| Institution: | 1. Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia 3. Malaysian Palm Oil Board, 6 Persiaran Institusi, Bandar Baru Bangi, 43000, Kajang, Selangor, Malaysia 4. Laboratory of Growth Regulators, Faculty of Science, Palacky University and Institute of Experimental Botany ASCR, ?lechtitelu 11, 783 71, Olomouc, The Czech Republic 6. Centre of the Region Han?? for Biotechnological and Agricultural Research, Faculty of Science, Palacky University, ?lechtitel? 11, 783 71, Olomouc, The Czech Republic 2. Felda Biotechnology Centre, PT 23417, Lengkuk Technology, Persiaran Technology, Techpark @ Enstek, 71760, Bandar Enstek, Negeri Sembilan, Malaysia 5. Institute of Tropical Agriculture, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia |
| Abstract: | Comparative mineral and hormonal analyses were made on tissue culture derived truncated leaf syndrome and wild type oil palm seedlings. Mineral analysis confirmed that Boron, Zinc and chlorophyll levels were significantly lower in truncated leaf syndrome leaves than those of wild type. Hormonal analysis also revealed various cytokinin derivatives such as trans-zeatin, trans-zeatin riboside, trans-zeatin O-glucoside and trans-zeatin riboside 5??mono phosphate were significantly higher in truncated leaf syndrome leaves compared to wild type leaves. Brassinolide level was also significantly higher in truncated leaf syndrome leaves than those of the wild type. These observations suggest that the truncated leaf syndrome abnormality could be associated to high cytokinin and brassinosteroid production which affects the uptake of Boron and Zinc. |
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