SCM2, a tryptophan permease in Saccharomyces cerevisiae, is important for cell growth |
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Authors: | Xiao Hong Chen Zhixiong Xiao Molly Fitzgerald-Hayes |
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Affiliation: | (1) Department of Biochemistry and Molecular Biology, Program in Molecular and Cellular Biology, University of Massachusetts, 01003-4505 Amherst, MA, USA;(2) Present address: Dana-Farber Cancer Institute and Harvard Medical School, 02115 Boston, MA, USA |
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Abstract: | SCM2, a novel gene encoding a yeast tryptophan permease, was cloned as a high-copy-number suppressor of cse2-1. The cse2-1 mutation causes cold sensitivity, temperature sensitivity and chromosome missegregation. However, only the cold-sensitive phenotype of cse2-1 cells is suppressed by SCM2 at high copy. SCM2 is located on the left arm of yeast chromosome XV, adjacent to SUP3 and encodes a 65 kDa protein that is highly homologous to known amino acid permeases. Four out of five disrupted scm2 alleles (scm21-4) cause slow growth, whereas one disrupted allele (scm25) is lethal. Cells with both the scm21 and trp1-101 mutations exhibit a synthetic cold-sensitive phenotype and grow much more slowly at the permissive temperature than cells with a single scm21 or trp1-101 mutation. A region of the predicted SCM2 protein is identical to the partial sequence recently reported for the yeast tryptophan permease TAP2, indicating that SCM2 and TAP2 probably encode the same protein. |
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Keywords: | Saccharomyces cerevisiae Amino acid permeases Transport Tryptophan |
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