Measurement of cardiac mechanical function in isolated ventricular myocytes from rats and mice by computerized video-based imaging |
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Authors: | Jun Ren Loren E Wold |
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Institution: | (1) Department of Pharmacology, Physiology, and Therapeutics, University of North Dakota School of Medicine, 501 N. Columbia Road, 58203 Grand Forks, ND |
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Abstract: | Isolated adult cardiac ventricular myocytes have been a useful model for cardiovascular research for more than 20 years. With
the recent advances in cellular physiology and transgenic techniques, direct measurement of isolated ventricular myocyte mechanics
is becoming an increasingly important technique in cardiac physiology that provides fundamental information on excitation-contraction
coupling of the heart, either in drug intervention or pathological states. The goal of this article is to describe the isolation
of ventricular myocytes from both rats and mice, and the use of real-time beat-to-beat simultaneous recording of both myocyte
contraction and intracellular Ca2+ transients.
Published: December 11, 2001 |
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Keywords: | Indexing terms" target="_blank">Indexing terms myocyte contractility intracellular Ca2+ transients edge detection |
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