人工哺育期腹泻婴猴奇异变形杆菌的分离与鉴定

目的对一株人工哺育期引发恒河猴婴猴腹泻的奇异变形杆菌进行了鉴定,为实验猕猴疾病检测、鉴别诊断提供参考依据。方法通过培养特性、菌落形态、染色、生化试验和血清学诊断鉴别等检查,对分离菌株进行初步鉴定,同时,对分离菌株进行致病性试验及药敏试验。结果通过表型生物学特性鉴定,并结合血清学诊断鉴别方法,确证该分离菌株为奇异变形杆菌,应用药敏试验筛选出了高度敏感的抗菌药,控制了该病的继续发生,致病性试验证明,该分离菌株对小白鼠有高致病性。结论分离到的奇异变形杆菌是导致本次婴猴腹泻死亡的病原菌,该菌为条件致病菌,对实验猕猴和研究人员均有潜在的危害,尽管该菌不是国家标准要求排除的病原菌,但该菌引发的传染病将对动物实验造成严重影响,故应引起高度重视。

Isolation and Identification of Proteus mirabilis from Diarrheic Infant Rhesus Monkeys in the Period of Artificial Feeding

Objective To identify the P.mirabilis strain isolated from infant rhesus monkeys in the period of artificial feeding,and provide useful data for detection and identification of the bacteria in laboratory rhesus monkeys.Methods P.mirabilis strains isolated from infant rhesus monkeys were identified by cultural characteristics,colony morphology,staining,motility and biochemical test and serological test.Meanwhile,antibiotic sensitivity,virulence and toxin tests were conducted.Results The results of cultural characteristics,colony morphology,staining,biochemical and serological tests showed that the isolate was a strain of P.mirabilis.Effective therapeutic drugs were screened by antibiotic sensitivity test,and the disease was effectively controlled.Virulence tests showed that it is a highly pathogenic strain to mice.Conclusions The results of this study demonstrate that P.mirabilis isolated from infant monkeys is the pathogenic agent leading to their diarrhea and death.This bacteria strain is a conditional pathogen,which may cause some potential harm to researchers and laboratory rhesus monkeys.Although laboratory rhesus monkeys are allowed to be P.mirabilis carriers in our country,it is worth to pay high attention to the serious consequence of P.mirabilis infection,and it should be considered as an important issue.