A recombinant peroxisome proliferator response element-driven luciferase assay for evaluation of potential environmental obesogens |
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| Authors: | Ya-Fen Wang How-Ran Chao Chia-Hsin Wu Chu-Han Tseng Ya-Ting Kuo Tsui-Chun Tsou |
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| Affiliation: | (1) Department of Bioenvironmental Engineering, Chung Yuan Christian University, Chungli, 320, Taiwan;(2) Department of Environmental Science and Engineering, National Pingtung University of Science and Technology, Neipu, Pingtung, 912, Taiwan;(3) Laboratory of Molecular Toxicology, Division of Environmental Health and Occupational Medicine, National Health Research Institutes, Zhunan, Miaoli, 350, Taiwan; |
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| Abstract: | A recombinant Huh7-PPRE-Luc cell line for analyzing the peroxisome proliferator response element (PPRE)-driven luciferase activity was established. The cells exhibited a good dose–response induction in PPRE-driven luciferase activity by three subtypes of peroxisome proliferator-activated receptor (PPAR) agonists as well as by a retinoid X receptor agonist, 9-cis-retinoic acid. Among five environmental chemicals tested, benzyl butyl phthalate and bisphenol induced PPRE-driven luciferase activation in Huh7-PPRE-Luc cells and caused adipogenic differentiation of 3T3-L1 cells. This recombinant Huh7-PPRE-Luc cell line would be useful for screening potential environmental obesogens with PPAR activity. |
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