The ADE2 gene from Saccharomyces cerevisiae: sequence and new vectors |
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Authors: | A Stotz P Linder |
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Institution: | Department of Microbiology, Biozentrum, Basel, Switzerland. |
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Abstract: | We have determined the sequence of a DNA fragment encoding the ADE2 gene from Saccharomyces cerevisiae. A DNA fragment of 2241 bp capable of complementing ade2 mutations was modified so it is available as a single BglII fragment for use in yeast vectors or for gene disruptions. The minimal fragment codes for a putative protein which is highly similar to the protein encoded by the ADE6 gene from Schizosaccharomyces pombe and to the proteins encoded by the purEK operon of Escherichia coli. |
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