Modification of a catalase by glutaraldehyde |
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Authors: | R. N. Mishaeva L. R. Gudkin N. P. Kuznetsova |
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Affiliation: | (1) Institute of High-Molecular-Weight Compounds, Russian Academy of Sciences, St. Petersburg, 199004, Russia |
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Abstract: | Polycondensation of a catalase (EC 1.11.1.6) with glutaraldehyde in order to stabilize the quaternary structure of an enzyme, maintain its activity, and protect it from thermal denaturation was studied. Synthesis showed a superequivalent utilization of the aldehyde groups relative to the catalase amine groups, as a result of the formation of glutaraldehyde oligomers linked to the enzyme. |
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