| 添加物对D-核糖生物合成的影响 |
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| 引用本文： | 陈长华,付水林,肖青,石锋,邱蔚然,高淑红. 添加物对D-核糖生物合成的影响[J]. 工业微生物, 2000, 30(2): 19-22 |
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| 作者姓名： | 陈长华 付水林 肖青 石锋 邱蔚然 高淑红 |
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| 作者单位： | 华东理工大学生物反应器工程国家重点实验室,上海,200237 |
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| 摘 要： | 采用枯草杆菌Ｄ２０１转酮醇酶缺陷型突变株，研究菌种生长与Ｄ－核糖合成优化工艺。在培养剂中添加山梨醇、ＫＨ２ＰＯ４和芳香族氨基酸，发现对菌的生长有刺激作用，特别是山梨醇，它可缩短延迟期，促进菌的生长。此外，用葡萄糖酸钙部分代替葡萄糖能增加Ｄ－核糖的产量，用葡萄糖和葡萄糖酸的混合碳源合碳源发酵，Ｄ－核糖产量可达到５２ｇ／ｌ。
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| 关 键 词： | D－核糖 发酵 生物合成 产量 山利醇 葡萄糖酸 |
Effects of adding materials on D-ribose biosynthesis |
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| CHEN Chang-hua,FU Shui-lin,XIAO Qing,SHI Feng,QIU Wei-ran,GAO Shu-hong. Effects of adding materials on D-ribose biosynthesis[J]. Industrial Microbiology, 2000, 30(2): 19-22 |
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| Authors: | CHEN Chang-hua FU Shui-lin XIAO Qing SHI Feng QIU Wei-ran GAO Shu-hong |
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| Abstract: | The strain growth and D ribose synthesis by Bacillus subtilis strain D201, a transketolase deficient D ribose producing mutant was studied.It was discovered that adding sorbitol, aromatic amino acid or KH 2PO 4 would stimulate the growth of Bacillus subtilis strain D201. Specially, adding sorbitol could reduced lag phase. When D glucose was quantitatively replaced by D gluconic acid, the D ribose production was enhanced. Fermentation with mixed carbon sources of glucose and gluconic acid could make the yield of D ribose as high as 52g/l. |
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| Keywords: | D ribose carbon source fermentation |
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