| 生物分子相互作用分析法测定甜菜组织中的钙调素 |
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| 引用本文： | 陈贵华,张少英,赵军锋.生物分子相互作用分析法测定甜菜组织中的钙调素[J].植物生理学通讯,2011(11):1117-1120. |
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| 作者姓名： | 陈贵华 张少英 赵军锋 |
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| 作者单位： | [1]内蒙古农业大学农学院,呼和浩特010019 [2]河北师范大学生物科学学院,石家庄050000 |
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| 基金项目： | 国家自然科学基金“甜菜抗丛根病信号转导与调控机制的研究”（30760125）和现代农业产业技术体系建设专项资金（CARS-03-04）. |
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| 摘 要： | 以甜菜为实验材料，经甜菜坏死黄脉病毒（beet necrotic yellow vein virus, BNYVV）侵染后，用生物分子相互作用分析（biomolecular interaction analysis，BIA）技术测定植物组织中钙调素（calmodutin，CaM）含量，分析在BNYVV侵染下植物组织中CaM的变化。该方法可以实时检测生物分子之间的相互作用，受杂质影响小，可简化样品的前处理，能够快速高通量分析大量的蛋白质样品，灵敏度高，准确性好。
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| 关 键 词： | 分子相互作用 CaM 甜菜 |
Determination of Calmodulin in Beta vulgaris L. Tissues by Biomolecular Interaction Analysis |
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| CHEN Gui-Hua,ZHANG Shao-Ying,ZHAO Jun-Feng.Determination of Calmodulin in Beta vulgaris L. Tissues by Biomolecular Interaction Analysis[J].Plant Physiology Communications,2011(11):1117-1120. |
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| Authors: | CHEN Gui-Hua ZHANG Shao-Ying ZHAO Jun-Feng |
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| Institution: | 1 College of Agronomy, Inner Mongolia Agriculture University, Hohhot 010019, China; 2School of Life Sciences, Hebei Normal University, Shijiazhuang 050000, China ) |
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| Abstract: | The sugar beet （Beta vulgaris） were used as experimental material. The calmodulin content and its changes in tissues were determined by biomolecular interaction analysis （BIA） technology after infected by beet necrotic yellow vein virus （BNYVV）. The technology can detect the real-time interaction between mole- cules, less affected by impurity, simplify sample pre-treatment, and analysis a large number of high-throughpnt protein rapidly. The technology is high sensitivity and good accuracy. |
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| Keywords: | molecular interactions CaM sugar beet （Beta vulgaris） |
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