Changes in calcium-dependent membrane permeability properties in mitochondria of livers from arthritic rats |
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Authors: | Silva Pamelli Maria de Souza Tanabe Eduardo Hermoso Aparecida Pinto Munhos Bersani-Amado Ciomar A Bracht Adelar Ishii-Iwamoto Emy L Salgueiro-Pagadigorria Clairce Luzia |
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Affiliation: | Laboratory of Biological Oxidations, Department of Biochemistry, University of Maringá, Maringá, Brazil. |
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Abstract: | The involvement of the mitochondrial permeability transition pore (PTP) in the responses of mitochondria from adjuvant-induced arthritic rats to Ca(2+) addition was investigated. The respiratory activity, the Ca(2+)-induced osmotic swelling and the electrophoretic (45)Ca(2+) uptake were evaluated in the absence and in the presence of cyclosporin A (CsA), a well-known inhibitor of the mitochondrial PTP. The Ca(2+)-induced mitochondrial permeability transition (MPT) process occurred in mitochondria from arthritic rats even in the presence of a low Ca(2+) concentration. Whereas in the normal condition, the Ca(2+)-induced uncoupling of oxidative phosphorylation and osmotic swelling was observed in the presence of 10 or 20 microM Ca(2+) concentration, in the arthritic condition, these events occurred at 1.0 microM concentration. In addition, mitochondria from arthritic rats presented an impaired ability to accumulate (45)Ca(2+). All these effects were completely prevented by the administration of CsA. The results of the present study suggest that the higher sensitivity of mitochondria from arthritic rats to Ca(2+)-induced MPT may be an important factor in the pathogenesis of the arthritis disease. |
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Keywords: | mitochondria mitochondrial permeability transition calcium arthritis |
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