Transformation of Bacillus subtilis by electroporation |
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| Authors: | Michele Stephenson Paul Jarrett |
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| Affiliation: | (1) Institute of Horticultural Research, BN17 6LP Littlehampton, West Sussex, UK |
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| Abstract: | Summary Optimal conditions for the transformation of Bacillus subtilis by electroporation were achieved. Frequencies of greater than 105 transformants/μg of plasmid DNA were obtained for a number of strains and plasmids. Increased transformation efficiency of mini-prep DNA from B. subtilis and Escherichia coli was obtained after microdialysis. |
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