The role of the lysines in the alkaline heme-linked ionization of ferric cytochrome c. |
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Authors: | G W Pettigrew I Aviram A Schejter |
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Institution: | The Institute of Medical Science, The University of Tokyo, P.O. Takanawa, Tokyo 108 Japan |
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Abstract: | Phage ?15 adsorbed at a low temperature (or by short-time incubation) to the outer surface of gathers on further incubation at a high temperature to a certain region where the inner and outer membranes may join. This was demonstrated by separating the inner and outer membranes of the cells in sucrose gradient after addition of 35S-labeled ?15 to the cells. Radioactivity adsorbed at 4° was first recovered mainly with the dense outer membrane but disappeared by further incubation at 35° within 5 min. Instead, the radioactivity was recovered with the membrane fraction which had intermediate density. Such phage translocation was not observed when phage ?15 was added to a to which the phage can adsorb but fail to infect. A host range mutant phage which can infect the mutant migrated to the intermediate dense region. |
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Keywords: | TNBS 2 4 6 trinitrobenzenesulfonic acid SDS sodium dodecyl sulfate |
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