Replica plating of mammalian cells using low melt agarose |
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Authors: | Shobhana Natarajan Tracy T Chow Jerry W Shay Woodring E Wright |
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Institution: | (1) Department of Cell Biology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX 75390, USA |
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Abstract: | We have developed a technique to replica plate mammalian cells grown on plastic dishes using low melt agarose. This method
is simpler than previously described methods that use polyester membranes to grow and transfer cells. We have tested the effectiveness
of this technique on normal and immortal cell lines and have found that we can transfer cells with an efficiency of 80–90%.
We have used this technique to rapidly screen clones for insertion of a lentivirally encoded gene without a selectable marker. |
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Keywords: | Cell culture Replica plating Ring cloning Screening Transfection |
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