In Vitro Preservation of Asparagus Officinalis |
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Authors: | SA Bekheet |
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Institution: | (1) Plant Cell and Tissue Culture Department, Genetic Engineering and Biotechnology Division, National Research Centre, El-Tahrir Str., Dokki, 12622, Cairo, Egypt |
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Abstract: | A simple systems for in vitro storage of health asparagus germplasm was developed. High percent (90 %) of shoots cultured in a standard multiplication medium were maintained viable in vitro at 5 °C in darkness for 12 months. This percent was decreased to 60 % when cultures were stored for 18 months. At normal temperature, shoots and callus cultures also survived for 1 year under osmotic stress on medium containing 40 g dm-3 mannitol. |
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Keywords: | cryopreservation mannitol osmotic stress |
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