Detection of Naegleria sp. in a thermal, acidic stream in Yellowstone National Park |
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Authors: | Sheehan Kathy B Ferris Michael J Henson Joan M |
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Affiliation: | Thermal Biology Institute, Montana State University, Bozeman, Montana 59717, USA. umbks@montana.edu |
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Abstract: | An initial survey of sequences of PCR-amplified portions of the 18S rRNA genes from a community DNA clone library, prepared from an algal mat in a thermal, acidic stream in Yellowstone National Park, WY, USA, revealed among other sequences, several that matched Vahlkampfia. This finding prompted further investigation using primers specific for Naegleria. Sequences from a subsequent DNA clone library, prepared from the 5.8S rRNA gene and the adjacent internal transcribed spacer (ITS) regions of the rRNA, closely matched Naegleria and formed an independent lineage within a clade containing Naegleria sturti and Naegleria niuginiensis. The sequences may represent a new Naegleria species. |
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Keywords: | Amoebic meningoencephalitis phylogenetics ribosomal RNA Vahlkampfiidae |
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