Smoke treatment triggers the release of a novel DNA damaging factor by lymphocytes |
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Authors: | Raghavendra Pralhada Rao S S Thammanna Gowda Srinivas Leela |
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Institution: | (1) Indian Institute of Science, Bangalore, 12, Karnataka, India;(2) Adichunchanagiri Cancer Research Institute, B.G. Nagara, 571 448 Bangalore, Karnataka, India;(3) Adichunchanagiri Biotechnology and Cancer Research Institute, B.G. Nagara, Nagamangala Talluk, Mandya District, 571 448 Bangalore, Karnataka, India |
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Abstract: | Abstact Organic fuel smoke is a hazardous agent, which pushes the cells towards“prooxidant state', leading to 4,46,400 strand breaks/cell/day
as against 47,000 strand breaks/cell/day produced by constitutive oxygen radicals. This prooxidants scenario switches on a
plethora of intercellular events. Here we report a novel DNA damaging factor released by lymphocytes, upon treatment with
smoke condensate. Human lymphocytes, when exposed to cow dung cake smoke condensate, were found to release a low molecular
weight factor into the media at 20 min of exposure. The conditioned media, displayed a propensity of inducing DNA damage in
fresh, normal lymphocytes, which were not exposed to any damaging agent. The above DNA damaging effect of the conditioned
media was not due to any residual presence of Polycyclic Aromatic Hydrocarbons, which were present in the smoke. The release
of this factor was in correlation with the DNA damaging event, taking place in the cells. This secondary DNA damaging factor
had a molecular weight less than 5 kd. The factor had the cell death inducing propensity when allowed to act on lymphocytes |
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Keywords: | DNA damage smoke condensate turmerin cell death |
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