Activation of the alternative complement pathway and generation of stimulating factors for granulocytes by glass fibers |
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Authors: | Mario Governa Matted Valentino Isabella Visona Isabella Marchiseppe Nicola Lo Martire |
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Institution: | (1) Institute of Occupational Medicine, University of Ancona, Torrette di Ancona, Italy;(2) Clinica di Medicina del Lavoro, Nuovo Ospedale Regionale, 60020 Torrette di Ancona, Italy;(3) Present address: ENICHEM, 160097 S. Donato Milanese, MI, Italy |
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Abstract: | Continuous-filament glass fibers coated with organic agents, candidate asbestos substitutes, were assessed for their ability to elicit from normal human serum complement-derived cleavage products which are able to stimulate the chemotaxis and the respiratory burst of polymorphonuclear leukocytes. Glass fibers generated chemoattracting and respiratory stimulating factors for polymorphonuclears from human serum. The effect was dose related for chemotaxis from the serum fiber concentration of 75 g/ml to 1,250 g/ml. The serum chemoattracting activity, as well the respiratory stimulation, were dramatically impaired when serum had been preliminarily absorbed with antiC5 antiserum. Since the impairment of chemotactic activity occurred also in the presence of EDTA, but not in the presence of EGTA, we assumed an activation of the alternative complement pathway.Glass fibers were studied in comparison to a UICC sample of Canadian chrysotile asbestos, which is able to activate in vitro the alternative complement pathway.Glass fibers exhibited less ability than asbestos fibers to generate complement cleavage products with chemotactic activity for polymorphonuclears; however, they produced an activity about equal to 80% of a chemotactic standard stimulus such as zymosan-activated plasma.Abbreviations AF
asbestos fibers
- antiCS-abs-S
NHS absorbed with antiserum against C5
- EDTA-CH-S
NHS treated with EDTA
- EGTA-Ch-S
NHS treated with EGTA
- GF
continuous filament glass fibers coated with a binder of organic substances
- NHP
normal human plasma
- NHS
normal human serum
- PMN
polymorphonuclear luekocytes
- ZAP
zymosan-activated plasma |
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Keywords: | asbestos fibers chemotaxis complement activation glass fibers granulocytes respiratory burst |
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