Detection of an NAD+-dependent malic enzyme locus in the atlantic salmonSalmo salar and other salmonid fish |
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| Authors: | Eric Verspoor William C Jordan |
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| Institution: | (1) SOAFD Marine Laboratory, P.O. Box 101, AB9 8DB Aberdeen, Scotland;(2) Division of Environmental and Evolutionary Biology, School of Biology and Biochemistry, The Queen's University, BT7 1NN Belfast, Northern Ireland;(3) Present address: Department of Biology, University of Southwestern Louisiana, 70504 Lafayette, Louisiana |
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| Abstract: | Electrophoretic studies of malate oxidoreductases routinely assess variation in two enzymes, malate dehydrogenase (EC 1.1.1.37) and malic enzyme (NADP+) (EC 1.1.1.40). By modification of the standard isozyme staining conditions for these enzymes, we have resolved a new NAD+-preferring, MgCl2-requiring malic enzyme which is indicated to be EC 1.1.1.39. The enzyme was detected in 10 salmonid fish species of the generaSalmo, Salvelinus, andOnchoryhncus. Phenotypic variation indicates that the novel enzyme is tetrameric and coded by a single locus. Inheritance inS. salar follows a single-locus model and the phenotypes are unlinked to polymorphisms fors
MDH-3,4* andm
MEP-2*, two malate oxidoreductase loci previously shown to be variable in this species.This work was supported by a contract to E. V. from Fisheries and Oceans Canada, St. John's, Newfoundland, and a postgraduate award to W. C. J. from the Department of Education for Northern Ireland. |
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| Keywords: | malic enzyme NAD+ polymorphism Salmo salmonids |
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