|
|||||
|
|
The Effect of Cork Pieces on Pseudohypericin Production in Cells ofHypericum perforatumShoots |
|
| Authors: | Kirakosyan A B Vardapetyan R R Charchoglyan A G Yamamoto H Hayashi H Inoue K |
| Institution: | (1) Department of Biophysics, Faculty of Biology, Yerevan State University, ul. A. Manukyana 1, Yerevan, 375025, Armenia;(2) Medical Plant Garden, School of Pharmaceutical Sciences, Nagasaki University, 1-14, Bunko-machi, Nagasaki, 852-8521, Japan;(3) Department of Pharmacognosy, Gifu Pharmaceutical University, 5-6-1 Mitahora-higashi, Gifu, 502-8585, Japan |
| Abstract: | The stimulating effect of cork pieces on hypericin and pseudohypericin biosyntheses was studied in cells of shoots regenerated from the callus cultures of St. John's wort (Hypericum perforatumL.). The addition of the cork matrix slightly stimulated shoot growth and enhanced pseudohypericin biosynthesis about threefold (to 0.4 mg/g dry wt). Pseudohypericin production increased proportionally with the amount of cork material added (from 1 to 4 mg/ml of growth medium). Further increase in the amount of cork pieces inhibited both pseudohypericin production and shoot growth. Organic and aqueous extracts of cork pieces did not affect the production of these substances. |
| Keywords: | Hypericum perforatum shoots hypericin pseudohypericin cork pieces stimulation |
| 本文献已被 SpringerLink 等数据库收录！ | |