Evaluating osteochondral defect repair potential of autologous rabbit bone marrow cells on type II collagen scaffold |
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Authors: | Wei-Chuan Chen Chao-Ling Yao Yu-Hong Wei I-Ming Chu |
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Institution: | (1) Department of Chemical Engineering, National Tsing Hua University, 101, Kuang-Fu Road, Hsin-Chu City, 300, Taiwan;(2) Department of Chemical Engineering and Materials Science, Yuan Ze University, Chung-Li, Taoyuan, 320, Taiwan;(3) Graduate School of Biotechnology and Bioengineering, Yuan Ze University, Chung-Li, Taoyuan, 320, Taiwan; |
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Abstract: | The feasibility of using genipin cross-linked type II collagen scaffold with rabbit bone marrow mesenchymal stem cells (RBMSCs)
to repair cartilage defect was herein studied. Induction of RBMSCs into chondrocytic phenotype on type II collagen scaffold
in vitro was conducted using TGF-β 3 containing medium. After 3-weeks of induction, chondrocytic behavior, including marker
genes expression and specific extracellular matrix (ECM) secretion, was observed. In the in vivo evaluation experiment, the
scaffolds containing RBMSCs without prior induction were autologous implanted into the articular cartilage defects made by
subchondral drilling. The repairing ability was evaluated. After 2 months, chondrocyte-like cells with lacuna structure and
corresponding ECM were found in the repaired sites without apparent inflammation. After 24 weeks, we could easily find cartilage
structure the same with normal cartilage in the repair site. In conclusion, it was shown that the scaffolds in combination
of in vivo conditions can induce RBMSCs into chondrocytes in repaired area and would be a possible method for articular cartilage
repair in clinic and cartilage tissue engineering. |
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Keywords: | Cartilage defect Type II collagen scaffold Rabbit bone marrow mesenchymal stem cells Chondrocyte-like cells In vivo |
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