Biodegradation of the sulfonylurea herbicide chlorimuron-ethyl by the strain Pseudomonas sp. LW3 |
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| Authors: | Ji-Ping Ma,Zhe Wang,Peng Lu,Hui-jie Wang,Shinawar Waseem Ali,Shun-Peng Li,& Xing Huang |
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| Affiliation: | Graduate School of Applied Chemistry, Shibaura Institute of Technology, Tokyo, Japan;;Department of Soil Science, Faculty of Agriculture, University of Gadjah Mada, Bulaksumur, Yogyakarta, Indonesia;and;Graduate School of Regional Environment Systems, Shibaura Institute of Technology, Tokyo, Japan |
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| Abstract: | Eleven carbazole (CAR)-degrading bacterial strains were isolated from seawater collected off the coast of Japan using two different media. Seven isolates were shown to be most closely related to the genera Erythrobacter, Hyphomonas, Sphingosinicella, Caulobacter , and Lysobacter . Meanwhile, strains OC3, OC6S, OC9, and OC11S showed low similarity to known bacteria, the closest relative being Kordiimonas gwangyangensis GW14-5 (90% similarity). Southern hybridization analysis revealed that only five isolates carried car genes similar to those reported in Pseudomonas resinovorans CA10 ( car CA10) or Sphingomonas sp. strain KA1 ( car KA1). The isolates were subjected to GC-MS and the results indicated that these strains degrade CAR to anthranilic acid. |
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| Keywords: | carbazole biodegradation marine bacteria |
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