Plasminogen activator activity in differentiating leukemia cells |
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Authors: | M Kidron I Nachshon M Mayer E Fibach |
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Affiliation: | 1. Departments of Biochemistry, Hebrew University-Hadassah Medical School, PO Box 24035, Jerusalem 91240, Israel;2. Hematology, Hadassah University Hospital, Mount Scopus, PO Box 24035, Jerusalem 91240, Israel |
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Abstract: | Plasminogen activator (PA) activity of human promyelocytic leukemia cell line HL-60 was assayed by following the conversion of plasminogen to plasmin and the plasmin-mediated hydrolysis of 14C-labeled globin. When HL-60 cells were induced to differentiate into macrophages by 12-O-tetradecanoyl-phorbol-13-acetate (TPA), cell-associated PA activity and secretion of PA into the conditioned medium increased profoundly. PA activity increased earlier and as a result of lower concentrations of TPA than the ability of the cells to adhere. Exposure to 10(-6)M dexamethasone did not prevent TPA-induced adherence and produced a slight inhibition of cellular PA activity. These findings imply that TPA-induced differentiation of HL-60 cells to macrophage-like cells is associated with induction of PA activity. |
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Keywords: | Plasminogen activator Myeloid leukemic cell Differentiation Macrophage PA, plasminogen activator |
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