不同培养方式对牛樟芝活性组分的影响

牛樟芝因含有多种活性成分且在抗癌、保肝等方面有着良好的功效而备受关注。本文考察了固态发酵以及不同液态发酵方式对牛樟芝活性产物的影响。结果显示,固态发酵不同谷物对活性产物的合成有明显的影响,青稞作为发酵谷物基质,其活性产物种类多且含量高,Antrodin C、Antroquinonol产量分别为5 901.27 mg/kg、3 715.76 mg/kg。常规液态发酵菌丝体中主要含有Antrodins类化合物,以Antrodin C为主,但不能合成Antroquinonol类化合物。通过添加前体物辅酶Q_0可以诱导牛樟芝菌丝体在液态发酵中合成Antroquinonol;在此基础上,添加植物油进行同步萃取发酵,能够显著提高牛樟芝菌丝体活性成分种类和产量,除了诱导合成Antroquinonol之外,还有大量Antroquinonol B合成;同时,大多数活性成分在发酵过程中不断被富集萃取,Antrodin C、Antroquinonol产量分别为289.80 mg/L、95.39 mg/L。Antrodin C产量比常规液态发酵提高了7.06倍,而Antroquinonol产量比诱导发酵提高了203.89%。

Influences of cultivation methods on active components of Antrodia camphorata

More attention was paid to Antrodia camphorata due to its anti-cancer and hepatoprotective effects.In this study,the influences of solid-state fermentation and different submerged fermentation methods on the active components of A.camphorata were investigated.The results showed that fermented grain types had obvious effects on the synthesis of active products.HighLand barley was the best substrate with high various active components.As a result,the yields of antrodin C and antroquinonol were as high as 5 901.27 mg/kg and 3 715.76 mg/kg,respectively.In conventional submerged fermentation,the antrodins compounds,mainly antrodin C,were in the mycelia of A.camphorata.However,antroquinonol compounds could not be produced.By adding its precursor of coenzyme Q0,antroquinonol could be biosynthesized in the submerged fermentation.Therefore,the vegetable oil was added during the synchronous extractive fermentation,significantly improving the active component types and yield.Besides antroquinonol,great amount of antroquinonol B was synthesized.Most active ingredients were enriched and extracted continually,among them the antrodin C and antroquinonol production reached 289.80 mg/L and 95.39 mg/L,respectively.The antrodin C production was increased by 7.06 times by comparing to that of the conventional submerged fermentation,while the antroquinonol production was increased by 203.89％ by comparing to that of the inducing fermentation.