| 耐热真菌的分类鉴定及rDNA-ITS系统发育分析 |
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| 引用本文： | 梁茵,王芳,李安娜,李多川. 耐热真菌的分类鉴定及rDNA-ITS系统发育分析[J]. 菌物学报, 2011, 30(4): 542-550 |
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| 作者姓名： | 梁茵 王芳 李安娜 李多川 |
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| 作者单位： | 山东农业大学植物病理学系,泰安,271018 |
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| 基金项目： | 国家自然科学基金“高温真菌多样性与分类研究”（No. 30870006） |
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| 摘 要： | 分别从山东、山西、河北、陕西、河南、云南、江西、海南、新疆、内蒙古、甘肃、广州等地实地采集堆肥、培养料、草堆等标本600多份，分离鉴定出耐热真菌28种，其中包括2个中国新记录种，沙生梭孢壳Thielavia arenaria和丰孢木霉Trichoderma saturnisporum，其余26个为中国已知种。在形态分类的基础上，对所分离鉴定的28株耐热真菌及购买的3株耐热真菌，进行rDNA-ITS序列测定，所测序列片段长度为554－895bp。将所测的31个和从GenBank下载的15个耐热真菌的rDNA-ITS序列构建NJ系统发育树，该树较好地显示了耐热真菌的系统进化关系，有力地支持了形态学的观点。
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| 关 键 词： | 高温真菌 中国新记录种 NJ系统发育树 |
Thermotolerant fungi and their phylogenetic analyses based on rDNA-ITS sequences |
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| LIANG Yin,WANG Fang,LI An-Na and LI Duo-Chuan. Thermotolerant fungi and their phylogenetic analyses based on rDNA-ITS sequences[J]. Mycosystema, 2011, 30(4): 542-550 |
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| Authors: | LIANG Yin WANG Fang LI An-Na LI Duo-Chuan |
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| Abstract: | More than 600 specimens of thermotolerant fungi were collected from 12 provinces in China,and 28 species were identified.Among them,Thielavia arenaria and Trichoderma saturnisporum are newly recorded species for China.rDNA-ITS sequencing was performed of the 28 species of thermotolerant fungi and additional 3 isolates purchased from China General Microbiological Culture Collection Center.The length of sequenced fragments were 554-895bp.Neighbor-joining phylogeny tree of rDNA-ITS sequences of the 31 isolates... |
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| Keywords: | fungi in high temperature new records for China NJ phylogeny tree |
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