山野木层孔菌子实体中抑制H22荷瘤小鼠肿瘤的活性成分研究

采用梯度提取法对山野木层孔菌子实体进行提取，得到石油醚层、甲醇层及水层3 种提取物及石油醚层中获得化合物4,6,8(14),22(23)-四烯-3-酮-麦角甾烷，并采用H22 荷瘤小鼠进行体内抗肿瘤活性研究，以抑瘤率、免疫器官指数、免疫因子为指标检测抗肿瘤活性。结果表明，石油醚高剂量组（100mg/kg）、单体化合物中剂量组（7.5mg/kg）抑制率分别为62.21%、57.67%；脾指数、胸腺指数均高于对照组和环磷酰胺（CTX）组，白介素-2（IL-2）的含量明显高于对照组和环磷酰胺（CTX）组（P＜0.01）；肿瘤坏死因子-α（TNF-α）含量明显低于对照组（P＜0.01）。因此认为上述石油醚提取物和单体化合物对H22荷瘤小鼠肿瘤有抑制作用，并且均能改善小鼠的免疫功能。

Antitumor active constituent in fruiting body of Phellinus yamanoi against Hepatoma H22 cell

Three kinds of extracts were made from the fruiting body of Phellinus yamanoi by gradient extraction method using petroleum ether,methanol and water as extracting solution,and 4,6,8(14),22(23)-tetraen-3-one-ergostane was isolated from petroleum ether extract.The anti-tumor activities of the three extracts and the monomer compound against mice Hepatoma H22 were studied in vivo.Inhibition rates of tumor,immune organ indexes and the contents of immune factors were evaluated as indicators.The results showed tha...