Human mesenchymal adipose stromal cells from mature adipocyte fraction |
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| Authors: | Aušra Unguryte Eiva Bernotiene Algirdas Venalis |
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| Institution: | (1) Stem Cell Biology Laboratory, Pennington Biomedical Research Center, Louisiana State University System, 6400 Perkins Road, 70808 Baton Rouge, LA, USA;(2) Department of Orthopedics, Louisiana State University Health Science Center, 1542 Tulane Avenue, 70112, Box T6-7, New Orleans, LA, USA;(3) Division of Orthopaedic Surgery, Department of Surgery, Duke University Medical Center, 375 Medical Sciences Research Building, 27710, DUMC Box 3093, Durham, NC, USA;(4) Tulane Center for Gene Therapy, Tulane University Health Sciences Center, J. Bennett Johnston Building, 1324 Tulane Avenue, SL-99, 70112-2699 New Orleans, LA, USA;(5) Tulane National Primate Research Center, 18703 Three Rivers Road, 70433-8915 Covington, LA, USA |
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| Abstract: | More effective techniques should be employed for isolation of human mesenchymal stromal cells derived from adipose tissue
(ADSC), seeking to make adipose tissue biopsies smaller in volume and thus less invasive. In this study, we compared properties
of ADSC isolated by several different methods from the same samples of adipose tissue in order to enhance yields of potential
ADSC. The mature adipocyte fraction was investigated using the ceiling culture method, including both ceiling and bottom cell
fractions, and the control culture method with standard amount of medium. The results were also compared using the stromal
vascular fraction from the same samples. The most efficient was the bottom cell population isolated from the mature adipocyte
fraction by ceiling culture method. These cells readily differentiated into osteogenic, adipogenic and chondrogenic lineages
and, similar to stromal vascular fraction cells, displayed high proliferation potential. Cultures of mature adipocyte fractions
with standard amount of medium were considerably less effective. Mature adipocyte fractions yields large quantities of adipose-derived
stem cells that have properties comparable with stromal vascular fraction cells suitable for tissue regeneration, especially
when only small biopsies can be taken. |
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