Cloning and Characterization of Functional Trehalose-6-Phosphate Synthase Gene in Maize |
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Authors: | Wei Jiang Feng-Ling Fu Su-Zhi Zhang Ling Wu Wan-Chen Li |
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Institution: | (1) Maize Research Institute, Sichuan Agricultural University, Xinkang 36, Ya’an, Sichuan, 625014, China; |
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Abstract: | Trehalose is a non-reducing disaccharide of glucose that functions as a compatible solute in the stabilization of biological
structures under heat and desiccation stress in bacteria, fungi, and some “resurrection plants”. In the plant kingdom, trehalose
is biosynthesized by trehalose-6-phosphate synthase (TPS) and trehalose-6-phosphate phosphatase (TPP). Over-expression of
exogenous and endogenous genes encoding TPS and TPP is reported to be effective for improving abiotic stress tolerance in
tobacco, potato, tomato, rice, and Arabidopsis. On the basis of bioinformatics prediction, we cloned a fragment containing an open reading frame of 2,820 bp from maize,
which encodes a protein of 939 amino acids. Phylogenetic analysis showed that this gene belongs to the class I subfamily of
the TPS gene family. Analysis of conserved domains revealed the presence of a TPS domain and a TPP domain. Yeast complementation
with TPS and TPP mutants demonstrated that this protein has the activity of trehalose-6-phosphate synthase. Semi-quantitative RT-PCR and real-time
quantitative PCR indicated that the expression of this gene is upregulated in response to both salt and cold stress. |
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