Application of autologous periosteal cells for the regeneration of class III furcation defects in Beagle dogs |
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Authors: | Jun Jiang Xiaohong Wu Minkui Lin Nghiem Doan Yin Xiao Fuhua Yan |
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Institution: | (1) School and Hospital of Stomatology, Fujian Medical University, 246 Yangqiao Zhong Road, 350002 Fuzhou, Fujian, People’s Republic of China;(2) Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, QLD, Australia; |
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Abstract: | The aim of this study was to evaluate the healing of class III furcation defects following transplantation of autogenous periosteal
cells combined with β-tricalcium phosphate (β-TCP). Periosteal cells obtained from Beagle dogs’ periosteum explant cultures,
were inoculated onto the surface of β-TCP. Class III furcation defects were created in the mandibular premolars. Three experimental
groups were used to test the defects’ healing: group A, β-TCP seeded with periosteal cells were transplanted into the defects;
group B, β-TCP alone was used for defect filling; and group C, the defect was without filling materials. Twelve weeks post
surgery, the tissue samples were collected for histology, immunohistology and X-ray examination. It was found that both the
length of newly formed periodontal ligament and the area of newly formed alveolar bone in group A, were significantly increased
compared with both group B and C. Furthermore, both the proportion of newly formed periodontal ligament and newly formed alveolar
bone in group A were much higher than those of group B and C. The quantity of cementum and its percentage in the defects (group
A) were also significantly higher than those of group C. These results indicate that autogenous periosteal cells combined
with β-TCP application can improve periodontal tissue regeneration in class III furcation defects. |
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Keywords: | Autologous periosteal cells Alkaline phosphatase Class III furcation defects Periodontal regeneration Tissue engineering β-Tricalcium phosphate |
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