Effect of papulacandin B on β-glucan synthesis in Schizosaccharomyces pombe |
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Authors: | Rosa Varona Pilar Pérez Angel Durán |
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Affiliation: | Department of Microbiology, Faculty of Biology, University of Salamanca, Spain;Institute of Microbial Biochemistry CSIC Salamanca, Spain |
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Abstract: | Abstract The antifungal antibiotic papulacandin β inhibited B(1,3)glucan-synthase activity, in vitro, from Schizosaccharomyces pombe . Levels of β(1,3)glucan-synthase from antibiotic-treated cultures were lower than the control cultures whereas mannan-synthase and β(1,3)glucanase activities were almost unaffected. The presence of an osmotic stabilizer reduced the inhibition of growth caused by the antibiotic. Addition of papulacandin β to a culture of S. pombe specifically inhibited incorporation of glucose into the β-glucan cell wall fraction. The fatty acids as well as the hydroxyl groups on the phenol residue of the papulacandin β molecule were essential for the inhibitory activity. |
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Keywords: | Antifungal cell wall fission yeast β(1 3)-glucan synthase inhibition |
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