Histone phosphorylation in late interphase and mitosis |
| |
Authors: | L R Gurley R A Walters R A Tobey |
| |
Affiliation: | Biomedical Research Group, Los Alamos Scientific Laboratory University of California, Los Alamos, New Mexico 87544 USA |
| |
Abstract: | Histone phosphorylation in late interphase has been investigated employing cells synchronized by the isoleucine-deprivation method, followed by resynchronization at the boundary using hydroxyurea. Phosphorylation occurred in both f1 and f2a2 as cells synchronously entered S phase following removal of hydroxyurea. The relative rates of phosphorylation of both species of histone increased in G2-rich and metaphase-rich cultures. A small amount of histone f3 phosphorylation was also observed in M-rich cultures which was not seen in G1, S, or G2-rich cultures. It is concluded that f1 phosphorylation is not dependent on continous DNA replication. These experiments suggest consideration of the concept that f1 phosphorylation is initiated as a preparation for impending cell division. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|