Chloroplast DNA markers (cpSSRs,SNPs) for <Emphasis Type="Italic">Miscanthus,Saccharum</Emphasis> and related grasses (Panicoideae,Poaceae) |
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Authors: | Mariateresa de Cesare Trevor R Hodkinson Susanne Barth |
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Institution: | (1) Teagasc Crops Research Centre, Oak Park, Carlow, Ireland;(2) School of Natural Sciences, Trinity College Dublin, Dublin, D2, Ireland; |
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Abstract: | Miscanthus and Saccharum are closely related perennial C4 grasses. Miscanthus has recently attracted interest as a non-food crop for energy and fibre production. However, molecular genetic tools for
the selection of new Miscanthus genotypes and study of its genetic resources are limited. We have identified six chloroplast (plastid) marker loci,containing
both microsatellites (cpSSRs) and single nucleotide polymorphisms (SNPs) and developed primers to amplify and sequence these
regions. The primers were designed using the complete chloroplast genome sequence of sugarcane and were tested on a collection
of 164 Miscanthus genotypes and 14 related species of the subfamily Panicoideae. The cpSSR markers were highly polymorphic, with the number
of alleles ranging from 10 to 16 per locus. Within the six cpSSR marker loci, the hybrid M. ×giganteus exhibits virtually no cpDNA variation compared with its putative parents M. sinensis and M. sacchariflorus. These SNP markers enable the differentiation of most Miscanthus species and detect infraspecific variation suitable for defining cytoplasmic genepools of Miscanthus for breeding purposes. |
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