The Grouping of Strains of Pseudomonas syringae subsp. savastanoi by DNA Restriction Fingerprinting |
| |
| Authors: | L. Mugnai L. Giovannetti S. Ventura G. Surico |
| |
| Affiliation: | 1. Istituto di Patologia e Zoologia forestale e agraria, Università di Firenze, P.le delle Cascine 28, 50144 Firenze, Italy;2. Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche, Sezione Microbiologia Applicata, Università di Firenze, P.le delle Cascine 27, 50144 Firenze, Italy;3. Centro di Studio dei Microrganismi Autotrofi, C.N.R., P.le delle Cascine 27, 50144 Firenze, Italy |
| |
| Abstract: | A selected group of strains of Pseudomonas syringae subsp. savastanoi from olive, oleander and ash were compared with pathogenicity tests and with DNA restriction fingerprinting using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and silver staining. The strains from each host were distinguishable by their pathogenicity to the same host and to the other two plant species. A division into the same groups was obtained with unweighted pair-group method with averages (UPGMA) clustering of the data from genomic fingerprinting, even though high overall similarity between the strains also indicated that they formed a single, well characterized taxon. It seems clear that the subspecies savastanoi of P. syringae comprises at least 3 groups of strains that differ in their precise host range, in the nature of the symptoms induced on the individual hosts, and in their genomic profile. |
| |
| Keywords: | |
|
|
