Immunolocalization of Cytosolic Phytochrome in the Green Alga Mougeotia*

The monoclonal antibody Z-3Bl (Schneider-Poetsch et al., Planta 173 1988] 61–72) was used to localize phytochrome in the Mougeotia cell by indirect immunofluorescence. The antibody detected a protein of about 110 kD in the immunoblot. Immunofluorescence indicated abundant phytochrome throughout the cytoplasm. In Mougeotia, a large cytoplasmic pool of phytochrome is presumably in equilibrium with a probably reactant-bound P_fr-pool at the plasmalemma, the latter being responsible for the physiological response.