Yak (Bos Grunniens) Stomach Lysozyme: Molecular Cloning,Expression and its Antibacterial Activities |
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| Authors: | Mingfeng Jiang Yan Chen Yong Wang Juan J Loor Yuhui Ye Yongli Wen |
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| Institution: | 1. Sichuan Key Laboratory of Conservation and Utilization of Animal Genetic Resources in Tibetan Plateau, Southwest University for Nationalities , Chengdu City, China mingfengjiang@vip.sma.com;3. Sichuan Key Laboratory of Conservation and Utilization of Animal Genetic Resources in Tibetan Plateau, Southwest University for Nationalities , Chengdu City, China;4. Department of Animal Sciences and Division of Nutritional Sciences , University of Illinois at Urbana-Champaign , Illinois, USA |
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| Abstract: | The cDNA coding for stomach lysozyme in yak was cloned. The cloned cDNA contains a 432 bp open reading frame and encodes 143 amino acids (16.24 KDa) with a signal peptide of 18 amino acids. Further analysis revealed that its amino acid sequence shares many common properties with cow milk lysozyme. Expression of this gene was also detected in mammary gland tissue by RT-PCR. Phylogenetic relationships among yak stomach lysozyme and 8 cow lysozymes indicated that the yak enzyme is more closely related to both cow milk lysozyme and the pseudogene ΨNS4 than cow stomach lysozyme. Recombinant yak lysozyme purified by Ni2+-column showed a molecular weight of 33.78 kDa and exhibited lytic activity against Staphylococcus aureus, providing evidence of its antibacterial activities. |
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| Keywords: | Expression Phylogenetic tree Stomach lysozyme Yak |
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